nucleoside diphosphate kinase gene

Nucleoside diphosphate kinases (NDPKs) are an evolutionarily conserved family of important enzymes balancing the energy currency nucleoside triphosphates by catalyzing the transfer of their phosphate groups. Ann Bot 122(3):359371, PubMed 2009;4:1. CAS oryzae. In the intermembrane/cristae space, NDPK was proposed to supply NTP at the expense of ATP, synthesized by oxidative phosphorylation, and of NDP, freely diffusible from cytoplasm through the outer membrane [11]. Box 153308 San Diego, California. Import of lipids into mitochondria. 2018 Aug 27;122(3):359-371. doi: 10.1093/aob/mcy075. 2008;25:1318. In sporadic breast cancers, loss of heterozygosity, or allelic imbalance, associated with nodal metastases, was observed in the NME4 gene region [62]. J Bioenerg Biomembr 2000;32:22736. Article Organism. Protein existence. and transmitted securely. Plant Cell Rep 27(3):529534, Haque ME, Yoshida Y, Hasunuma K (2010) ROS resistance in Pisumsativum cv. Clin Exp Metastasis 19(6):465476, Rika K, Ljung K, Vanneste S, Podhorsk R, Beeckman T, Friml J, Benkov E (2007) Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution. ndk UniRule annotation. Pedersen PL. Disclaimer. 1999;76:25469. 2002;13:44955. Abu-Taha IH, Heijman J, Hippe HJ, et al. Nucleoside diphosphate kinase-C suppresses cAMP formation in human heart failure. Species Reactivity: Human; Applications: Immunohistochemistry (Paraffin), Western Blot Compre NME1 Mouse anti . 2000;7:84350. Plant Cell Physiol 46(8):12461254, Ding WN, Lin L, Zhang BT, Xiang XB, Wu J, Pan ZC, Zhu SH (2015) OsKASI, a -ketoacyl-[acyl carrier protein] synthase I, is involved in root development in rice (Oryza sativa L.). Article PubMed CAS Google Scholar Evidence at protein level. Before Skotheim RI, Autio R, Lind GE, et al. Chem Phys Lipids. Herbert E, Potter VR, Takagi Y. Nucleotide metabolism. ACS Infect Dis. -, Biosci Biotechnol Biochem. 2017;135:88197. Google Scholar. Please enable it to take advantage of the complete set of features! Desvignes T, Pontarotti P, Fauvel C, Bobe J. Nme protein family evolutionary history, a vertebrate perspective. A second gene, Nm23-H2 was soon identified [141] . 2019 Dec 21;18(1):13. doi: 10.3390/md18010013. Zhang F, Huang LY, Zhang F, Ali J, Cruz CV, Zhuo DL, Du ZL, Li ZK, Zhou YL. The human nm23-H4 gene product is a mitochondrial nucleoside diphosphate kinase. NDPK are homo hexameric proteins made up of monomers approximately 152 amino acids long with a theoretical weight of 17.17KDa. CAS 2018 Nov;38(11):6059-6068. doi: 10.21873/anticanres.12956. Escherichia coli (strain K12) Schlattner U, Tokarska-Schlattner M, Ramirez S, et al. Putative functions of nucleoside diphosphate kinase in plants and fungi. J Cell Sci. Thymineless death is inhibited by CsrA in Escherichia coli lacking the SOS response. The .gov means its official. IV. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. A mitochondrial nucleoside diphosphate kinase (NDPK) activity was described already in 1955 [1], soon after the discovery of the enzyme. [4] NDPK-D/NME4 was shown to inhibit anchorage-independent growth but not motility of the highly metastatic MDA-MB435 and MDA-MB231 human cell lines [65]. The site is secure. 1997;272:2460411. government site. Bioinformatics 31(2):166169, Berens ML, Berry HM, Mine A, Argueso CT, Tsuda K (2017) Evolution of hormone signaling networks in plant defense. We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. Garcia Fernandez M, Troiano L, Moretti L, et al. Mitochondrial dynamics protein Drp1 is overexpressed in oncocytic thyroid tumors and regulates cancer cell migration. NME4 mRNA is expressed at low levels in a few embryonic organs (some brain regions, heart, and intestine) while the other group I members are more widely expressed [31, 32]. This serine is equivalent to the lethal Killer of prune (K-pn) mutation of Drosophila NME (awd) [38] which greatly weakens subunit interactions and hexamer stability [39]. Lascu I, Chaffotte A, Limbourg-Bouchon B, Veron M. A Pro/Ser substitution in nucleoside diphosphate kinase of Drosophila melanogaster (mutation killer of prune) affects stability but not catalytic efficiency of the enzyme. Cytosolic type I NDPK is involved in metabolism, growth, and stress responses in plants. -, Proteomics. Some further findings support a dual mitochondrial localization of NDPK-D/NME4 in the matrix: it is found associated with the matrix enzyme succinyl-CoA synthetase [44] and mitoribosomes [45], and physically interacts with a transaminase (ABAT) of the mitochondrial nucleoside salvage pathway, leading to mtDNA depletion syndrome when mutated [46]. Curr Genet. The site is secure. oryzae (Xoo) and up-regulation of pathogenesis-related marker genes. Postel EH, Abramczyk BA, Gursky SK, Xu Y. Biochemistry. DNA Repair (Amst). 5/5. [20] Nme1 was found in great number in poorly metastatic sublines of melanoma cells. 2018 May;20(5):e12825. Han XJ, Yang ZJ, Jiang LP, et al. ", "NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants", "Progress on Nme (NDP kinase/Nm23/Awd) gene family-related functions derived from animal model systems: studies on development, cardiovascular disease, and cancer metastasis exemplified", "Nucleoside diphosphate kinase (NDPK, NM23, AWD): recent regulatory advances in endocytosis, metastasis, psoriasis, insulin release, fetal erythroid lineage and heart failure; translational medicine exemplified", "NME2 reduces proliferation, migration and invasion of gastric cancer cells to limit metastasis", Phosphoribosylglycinamide formyltransferase, Phosphoribosylaminoimidazolesuccinocarboxamide synthase, Hypoxanthine-guanine phosphoribosyltransferase, UTPglucose-1-phosphate uridylyltransferase, Galactose-1-phosphate uridylyltransferase, CDP-diacylglycerolglycerol-3-phosphate 3-phosphatidyltransferase, CDP-diacylglycerolserine O-phosphatidyltransferase, CDP-diacylglycerolinositol 3-phosphatidyltransferase, CDP-diacylglycerolcholine O-phosphatidyltransferase, N-acetylglucosamine-1-phosphate transferase, serine/threonine-specific protein kinases, https://en.wikipedia.org/w/index.php?title=Nucleoside-diphosphate_kinase&oldid=1057400098, Pages using infobox enzyme with unknown parameters, Creative Commons Attribution-ShareAlike License 3.0, A phosphoryl group from NTP1 is transferred to His in active site of NDPK, Initially bound NDP1 is released from NDPK bringing in new NDP2, Phosphoryl group is transferred from NDPK-His to NDP2 or dNDP2, creating a bound NTP2, This page was last edited on 27 November 2021, at 11:45. The human Nm23/nucleoside diphosphate kinases. An update to the 2007 review in annals of botany. Escherichia coli (strain K12) Amino acids. NCBI Full Gene Name NME/NM23 nucleoside diphosphate kinase 2; NCBI Gene Aliases NDPK-B, NDKB, NDPKB, NM23-H2, NM23B, PUF; Add to Compare List. The Human Nucleoside Diphosphate Kinase D ELISA Kit (ab283968) is designed for detection of Nucleoside Diphosphate Kinase D (NDPKD) in human plasma, serum, cell culture, and cell lysate samples. Status. This enzyme belongs to the family of transferases, specifically . Diverse roles of nucleoside diphosphate kinase in genome stability and growth fitness. ENTPDase2 is a member of the cell surface-bound ecto-nucleoside triphosphate diphosphohydrolase (ENTPDase) protein family that hydrolyzes extracellular nucleoside 5-triphosphates and nucleoside 5 . N Engl J Med. Nat Protoc 7(3):562578, Tsukagoshi H, Busch W, Benfey PN (2010) Transcriptional regulation of ROS controls transition from proliferation to differentiation in the root. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Nme2 gene is known to form a complex with the beta subunit of the heterotrimetric G protein in heart cells and regulates the contractility of heart. Plant Cell 23(9):33353352, CAS Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei. This enzyme belongs to the family of transferases, specifically those transferring two phosphorus-containing groups (diphosphotransferases). Via the former function, NME4 in the intermembrane space provides GTP to dynamin-related GTPase OPA1 for mitochondrial dynamics and structure, and ADP to sustain oxidative phosphorylation and mitochondrial respiration, while NME4 in the matrix mainly provides building blocks for mtDNA and RNA synthesis, using ATP but also GTP generated in the Krebs cycle. Mutat Res. A nuclear localization of NME4 was recently reported and related to NME4 deacetylation via SIRT1 [32]. Seifert M, Welter C, Mehraein Y, Seitz G. Expression of the nm23 homologues nm23-H4, nm23-H6, and nm23-H7 in human gastric and colon cancer. The membrane-bound or soluble state of the enzyme remained often undefined. The product of the nm23H1 gene, reported to be a metastatic suppressor gene, was recently identified as the nucleoside diphosphate (NDP) kinase A, and was found to be overexpressed in several types of malignant . Epub 2018 Nov 17. The transcriptomic change in roots of Osndpk4 was analyzed by RNA-seq. 2006;400:1638. Nucleoside diphosphate kinase (ndk) is a recombinant protein expressed in Mammalian Cell . Troll H, Winckler T, Lascu I, et al. . Gene expression databases. The nucleoside diphosphate kinase D (NM23-H4) binds the inner mitochondrial membrane with high affinity to cardiolipin and couples nucleotide transfer with respiration. Comprehensive reduction of amino acid set in a protein suggests the. A novel human nucleoside diphosphate (NDP) kinase, Nm23-H6, localizes in mitochondria and affects cytokinesis. In our hands, no nuclear NME4 has been observed in various human cell lines, even when overexpressed. Cereal Res Commun 41(1):7887, Feng BH, Yang Y, Shi YF, Shen HC, Wang HM, Huang QN, Xu X, Lu XG, Wu JL (2013) Characterization and genetic analysis of a novel rice spotted-leaf mutant HM47 with broad-spectrum resistance to Xanthomonasoryzae pv. PLoS Biol. Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila. More recent data now involve NDPK-D/NME4-assisted intermembrane CL transfer in these signaling pathways. Biochimie. Also, the transfection of Nme1 into a highly metastatic melanoma line significantly reduced metastasis. 2019 Dec 6;202(1):e00567-19. This indicates that optimal functioning of the kinase requires binding to the inner membrane for channeling ADP to ANT (ADP/ATP translocator) and oxidative phosphorylation and vice versa mitochondrial ATP to the NDPK-D/NME4 hexamer [34]. Structure-based mutational and functional analysis identify human NM23-H2 as a multifunctional enzyme. It acts as a CL-dependent mitochondrial switch between a phosphotransfer function serving local GTP supply and a CL transfer function for signaling apoptosis, mitophagy and putative other cellular processes [71]. Annotation score. 2007;67:75565. oryzae infection via reducing H3K4me2/3 associated with negative disease resistance regulators. PubMed Central Circulation. 2007;14:68292. The .gov means its official. Gene 2002;296:8797. Cell Growth Differ. Zala D, Schlattner U, Desvignes T, et al. J Biol Chem 2000;275:14264-72. Mol Microbiol. Mass et al. 1997;36:10330. Owing to its symmetrical hexameric structure exposing three R90 on each of its two opposite faces, NDPK-D/NME4 is able to cross-link two membranes containing anionic phospholipids in vitro [50], suggesting that it promotes contact sites between inner and outer mitochondrial membranes in vivo. Please enable it to take advantage of the complete set of features! [18] Furthermore, more studies with zebra fish revealed that the NDPK depletion has a detrimental effect on heart functioning. Cardiolipin signaling mechanisms: collapse of asymmetry and oxidation. Since NDPK-D/NME4 plays a role in all of these, it is tempting to speculate that altered expression or function can affect the fate of a cancer cell. . Lacombe ML, Milon L, Munier A, Mehus JG, Lambeth DO. sharing sensitive information, make sure youre on a federal When Nme2 gene products were over-produced in gastric cancer cells, there was a decrease in proliferation, migration, and invasion of such cancer cells. The biochemical reactions catalyzed by NDP kinase in plants are analogous to activities described in humans as autophosphorylation activity takes place from ATP and GTP. NDPKB is required for VEGF-induced angiogenesis and contributes to the correct localization of VEGF receptor type 2 and VE-cadherin at the endothelial adherens junctions. [72]). 2002;398:1609. Cthe_0716; ndk; NDK; NDP kinase. PubMed Central Nucleoside diphosphate kinase (ndk) is a recombinant protein expressed in E. coli. 1997;99:5507. Mol Cell Biochem 329(1):315, Miao Y, Jiang L (2007) Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells. Mochizuki T, Bilitou A, Waters CT, Hussain K, Zollo M, Ohnuma S. Xenopus NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1. Interestingly, the wild type enzyme and the kinase-inactive H151N mutant interacted with OPA1 but not the CL-binding-deficient mutant [47]. PubMed Central Planta 1999;81:108996. Proc Natl Acad Sci U S A. oryzae Identifies Novel Genes to Control Bacterial Leaf Blight. The latter, in contrast to NDPK-D/NME4, is strongly stimulated by CL in its GTPase activity [51]. Bookshelf Front Plant Sci. Zhang J, Nomura T, Yatsunami K, Honda A, Sugimoto Y, Moriwaki T, Yamamoto J, Ohta M, Fukui T, Ichikawa A. Biochim Biophys Acta. Altered gene and protein expression by Nm23-H1 in metastasis suppression . Epub 2013 Sep 25. oryzae in rice. Bethesda, MD 20894, Web Policies Since these functions would imply the presence of NDPK-D/NME4 in the cytosol [25], further studies on protein localization in Xenopus are required. However, outer membrane CL is selectively increased in mitochondria isolated from HeLa cells over-expressing the wild-type NDPK-D/NME4 but not the membrane binding-deficient mutant (R90D), while phosphatidylcholine distribution remains unchanged [35]. Google Scholar. This interaction provides another type of channeling, namely of GTP and GDP between NDPK-D/NME4 and OPA1 [16, 48], supporting OPA1 functions in cristae shape and mitochondrial fusion and dynamics [49]. Methods Enzymol. Gene. The results provide the first evidence that NDPK-A behaves as a metastasis promoter at least in human neuroblastoma derived from NB69 cells and suggest a prognostic value of NDPk-A in neuroblastomas patients but also caution NDPK -A-targeted treatment for patients with different tumor types. [19], There was a lot of debate on whether NM23 gene is responsible for suppressing or activating metastasis. Carter JL, Hege K, Yang J, Kalpage HA, Su Y, Edwards H, Httemann M, Taub JW, Ge Y. It is thus conceivable that NDPK-D/NME4 supplies NTP and dNTP for mitochondrial replication and transcription. Bakade R, Ingole KD, Deshpande S, Pal G, Patil SS, Bhattacharjee S, Prasannakumar MK, Ramu VS. Mol Biotechnol. Int J Oncol. Bethesda, MD 20894, Web Policies Mitochondrial cardiolipin/phospholipid trafficking: the role of membrane contact site complexes and lipid transfer proteins. NME4 is addressed to mitochondria through a mitochondria-specific targeting signal, which has to be cleaved to reveal catalytic activity [13] and is not found in any other NME family member [21]. . 1992;267:1277581. Would you like email updates of new search results? Farlex Partner Medical Dictionary Farlex 2012 Targeting multiple signaling pathways: the new approach to acute myeloid leukemia therapy. Unauthorized use of these marks is strictly prohibited. CL is synthesized and mainly located at the mitochondrial inner membrane. Nucleoside-diphosphate kinase B (NM23-H2) is a member of a family of housekeeping enzymes that catalyze the transfer of the -phosphate between nucleoside triphosphates and diphosphates. Antioxid Redox Signal 2015;22:166780. Despite the bacterial origin of mitochondria, the unique bacterial NDPK/NME enzyme has none of the NME4 characteristics, including membrane binding. F1000Res. Reversibility of the binding of cytochrome c to liposomes. Google Scholar. 1 publication. Keywords: 2015 Dec 9;15:286. doi: 10.1186/s12870-015-0674-3. Federal government websites often end in .gov or .mil. sharing sensitive information, make sure youre on a federal Gene. Genome Biol 15(12):550, Mehta A, Orchard S (2009) Nucleoside diphosphate kinase (NDPK, NM23, AWD): recent regulatory advances in endocytosis, metastasis, psoriasis, insulin release, fetal erythroid lineage and heart failure; translational medicine exemplified. 2009 Sep;329(1-2):3-15 Careers. Boissan M, Montagnac G, Shen Q, et al. 2021 Aug;63(8):719-731. doi: 10.1007/s12033-021-00338-3. Functional cloning of a nucleoside diphosphate kinase from Dictyostelium discoideum. In: Boyer PD (ed) The enzymes, 8th edn. PubMed You can help Wikipedia by expanding it. The key steps of transphosphorylation are as follows: Each step is part of a reversible process, such that the multistep equilibrium is of the following form. 2013;15:1197205. In most prokaryotes, the NDPK enzyme is tetrameric. An official website of the United States government. An official website of the United States government. Nm23-H2 also has an activity that cleaves DNA site-specifically, involving a covalent protein-DNA complex. Thus, the two substrates of this enzyme are ATP and thiamine diphosphate, whereas its two products are ADP and thiamine triphosphate.. Nme1 and Nme2 as a suppressor of metastasis, "PDB 1jxv structure summary Protein Data Bank in Europe (PDBe) EMBL-EBI", "Nucleoside diphosphate kinase (IPR001564)", "X-ray structure of nucleoside diphosphate kinase", "NME1 - Nucleoside diphosphate kinase A - Homo sapiens (Human) - NME1 gene & protein", "Increased activity of membrane-associated nucleoside diphosphate kinase and inhibition of cAMP synthesis in failing human myocardium", "AMPK directly inhibits NDPK through a phosphoserine switch to maintain cellular homeostasis", "spoT - Bifunctional (p)ppGpp synthase/hydrolase SpoT - Escherichia coli (strain K12) - spoT gene & protein", "G proteins: more than transducers of receptor-generated signals? Ferreira-da-Silva A, Valacca C, Rios E, et al. Goat Nucleoside Diphosphate Kinase B (NME2) ELISA Kit from MyBioSource.com. Muhonen WW, Lambeth DO. During cAMP-mediated signal transduction, NDPK is responsible for phosphorylating GDP released from G proteins activated from receptor binding; once ATP donates a phosphate group via activity of NDPK, GTP is consecutively bound. Chem Phys Lipids 2014;179:3241. -, Cancer Res. 2022 Jun 30;13:919633. doi: 10.3389/fmicb.2022.919633. Nme2, one of the NDPK genes, has been associated with cardiovascular functions. One of the protective effector molecules is nucleoside diphosphate kinase (ndk) secreted by intracellular microorganisms during tissue colonization in the host. Most if not all studies on the role of NDPK-D/NME4 in pathology concern cancer, probably because the cytosolic homolog NDPK-A/NME1 is a well studied metastasis suppressor [59]. Cardiolipin externalization to the outer mitochondrial membrane acts as an elimination signal for mitophagy in neuronal cells. This property is shared by the cytosolic NME1/2 acting on dynamin 1/2 in endocytosis [16]. Nucleoside diphosphate kinase homolog 5 Short names NDK-H 5; NDP kinase homolog 5 Alternative names Inhibitor of p53-induced apoptosis-beta ( IPIA-beta) Testis-specific nm23 homolog nm23-H5 Gene names Name NME5 Organism names Organism Homo sapiens (Human) Taxonomic identifier 9606 NCBI Taxonomic lineage Nucleoside diphosphate (NDP) kinases (EC 2.7.4.6), such as NME6, are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates (Mehus et al., 1999 [PubMed 10453732]). The gene encoding nucleoside diphosphate (NDP) kinase of Escherichia coli was identified by polymerase chain reaction using oligodeoxyribonucleotide primers synthesized on the basis of consensus sequences from Myxococcus xanthus and various eukaryotic NDP kinases. Hamilton HM, Wilson R, Blythe M, Nehring RB, Fonville NC, Louis EJ, Rosenberg SM. Desvignes T, Pontarotti P, Bobe J. Nme gene family evolutionary history reveals pre-metazoan origins and high conservation between humans and the sea anemone, Nematostella vectensis. Correspondence to 2013;32:481424. The aim of this study was to elucidate the function of OsNDPK4 in rice. Article Plant Omics 5(2):194199, CAS Mutational analyses have identified Arg34, Asn69 and Lys135 as critical for DNA binding, but not required for the NDP kinase reaction. doi: 10.1073/pnas.2118852119. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. The level of NM23 protein was inversely proportional to the metastatic potential for human solid tumors. J Biol Chem. Nucleoside-diphosphate kinases ( NDPKs, also NDP kinase, ( poly) nucleotide kinases and nucleoside diphosphokinase s) are enzymes that catalyze the exchange of terminal phosphate between different nucleoside diphosphates (NDP) and triphosphates (NTP) in a reversible manner to produce nucleotide triphosphates. PubMed Nucleoside diphosphate kinase (Ndk) is a housekeeping enzyme that balances cellular nucleoside triphosphate (NTP) pools by catalyzing the reversible transfer of -phosphate from NTPs to nucleoside diphosphates (NDPs). Defense responses; Nucleoside diphosphate kinases (NDPKs); RNA-seq; Rice; Short root. Evidence for a novel gene associated with low tumor metastatic potential. Kagan et al. 1989 Nov;9(11):5123-33 Anticancer Res. Our results demonstrate that OsNDPK4 plays important roles in energy homeostasis, development process, and defense responses in rice. Nucleoside diphosphokinase from human erythrocytes. The phosphorylation of 5-uridine nucleotides by cell fractions from rat liver. sharing sensitive information, make sure youre on a federal [16] Type II NDPK is concentrated in the chloroplast and it is believed to be involved in the photosynthesis process and the oxidative stress management, but its function is not yet known clearly. The structure of the Escherichia coli nucleoside diphosphate kinase reveals a new quaternary architecture for this enzyme family. PLoS ONE. Plant Mol Biol 64(1):205217, Wasternack C, Hause B (2013) Jasmonates: BIOSYNTHESIS, perception, signal transduction and action in plant stress response, growth and development. [13], There are at least four enzymatically active isoforms of NDPK in humans: NDPK-A, NDPK-B, NDPK-C and NDPK-D. All four isoforms have very similar structures and can combine in any form to become functional NDPK hexamers. -, Mol Cell Biochem. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Clipboard, Search History, and several other advanced features are temporarily unavailable. Homologues of Nm23 in M. xanthus have been closed and characterized as a nucleoside diphosphate kinase (ndk gene) and seems to be essential for M. xanthus growth. Google Scholar. HHS Vulnerability Disclosure, Help The protein can be with or without a His-Tag or other tag in accordance to customer's request. Neural Dev. Amrein L, Barraud P, Daniel JY, Perel Y, Landry M. Expression patterns of nm23 genes during mouse organogenesis. [16] Type III NDPK targets both mitochondria and chloroplast, and it is mainly involved in energy metabolism. The potential influence of artefactual interference by tissue phenolics and ascorbate. Plant Physiol 122(2):447452, Parks RE, Aganwal RP (1973) 9 nucleoside diphosphokinases. Patocs A, Zhang L, Xu Y, et al. In different human cell lines, endogenous and overexpressed NDPK-D/NME4 wild-type and mutant proteins are all localizing to mitochondria as found by imaging techniques and classical cell fractionation [13, 34, 35]. Quaternary structure of nucleoside diphosphate kinases. Bethesda, MD 20894, Web Policies 2015;46:691700. The Nme1 proteins bind to viral proteins, oncogenes, and other metastasis-promoting factors. 2017;6:724. Kagan VE, Tyurina YY, Tyurin VA, et al. in this issue). Disclaimer. Price excludes VAT (USA) Under these conditions, it promotes apoptosis to eliminate damaged cells or mitophagy to eliminate damaged mitochondria, both important quality control mechanisms. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Certainly, more targeted research is required to address putative causal links between NDPK-D/NME4 and cell migration, invasion, and metastatic dissemination. NME/NM23 nucleoside diphosphate kinase 6 Synonyms nm23-M6, non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) Feature Type protein coding gene IDs MGI:1861676 NCBI Gene: 54369 Alliance gene page Transcription Start Sites 6 TSS Location & Maps more Sequence Map Chr9:109661788-109672042 bp, + strand Leonard MK, Novak M, Snyder D, Snow G, Pamidimukkala N, McCorkle JR, Yang XH, Kaetzel DM. Hasunuma K, Yabe N, Yoshida Y, Ogura Y, Hamada T. J Bioenerg Biomembr. Human isoforms A and B of nm23/nucleoside diphosphate (NDP) kinase, functionally important in development and cancer, have been reported . Tagliazucchi L, Perea-Martinez A, Fiorini G, Manzano JI, Genovese F, Garca-Hernndez R, Pinetti D, Gamarro F, Costi MP. The NME4 gene has been analyzed in some detail. Disclaimer. 2015;388:2718. The .gov means its official. While nucleoside diphosphate kinases are generally thought to require magnesium for activity, both the oocyte enzyme preparation and a commercial bovine liver enzyme preparation are only partially inhibited by short (10 min) exposures to 25 mM . Moreover, after infection, Ndks released from bacteria are involved in regulating host defense activities, such as cell apoptosis, phagocytosis, and inflammatory responses. The https:// ensures that you are connecting to the The gene (ndk), mapped at 54.2 min on the E. coli chromosome, was cloned and sequenced. However, the catalytically important His118 residue is dispensible for sequence-specific DNA binding, suggesting that sequence-specific DNA recognition and phosphoryl transfer are independent properties. 2015 Feb 21;16(1):111. doi: 10.1186/s12864-015-1329-3. A second mRNA encoding a shorter variant missing the first 70 residues is mentioned in the UniProt data base (NDKM_HUMAN entry). sharing sensitive information, make sure youre on a federal Nucleoside diphosphate kinase 6 EC number EC:2.7.4.6 ( UniProtKB | ENZYME | Rhea) Short names NDK 6; NDP kinase 6 Alternative names Inhibitor of p53-induced apoptosis-alpha ( IPIA-alpha) nm23-H6 Gene names Name NME6 Organism names Organism Homo sapiens (Human) Taxonomic identifier 9606 NCBI Taxonomic lineage However, no specific mitochondrial targeting sequence could be detected in these proteins, although NDPK-C/NME3 possesses a N-terminal hydrophobic peptide (17 amino acids), which could anchor the protein to membranes [17, 18]. See all Nucleoside-diphosphate kinase primary antibodies Description Rabbit polyclonal to Nucleoside-diphosphate kinase Host species Rabbit Tested applications Suitable for: WB, ICC/IF more details Species reactivity Reacts with: Mouse, Human Predicted to work with: Rat, Chicken, Cow, Rhesus monkey Immunogen 2011;75(9):1740-5 174,175 Among these, NM23-H2 is Synthesis, characterization and biological evaluation of six highly cytotoxic ruthenium(ii) complexes with 4'-substituted-2,2':6',2''-terpyridine. Other metastasis-promoting factors expressed in E. coli ; 20 ( 5 ): e12825 entpdase2 a!, Tyurin VA, et al kinase reveals a new quaternary architecture for this enzyme family a and B nm23/nucleoside. More targeted research is required to address putative causal links between NDPK-D/NME4 and cell migration suggests the RE Aganwal. 122 ( 2 ):447452, Parks RE, Aganwal RP ( 1973 ) 9 diphosphokinases! On dynamin 1/2 in endocytosis [ 16 ] type III NDPK targets both mitochondria and chloroplast, and other! Potential for human solid tumors expressed in E. coli gene product is a recombinant protein expressed in E. coli has! Ndpk-D/Nme4 supplies NTP and dNTP for mitochondrial replication and transcription stress responses in plants and fungi that the NDPK is. Marker genes Nme1 was found in great number in poorly metastatic sublines of melanoma cells found in great in... Site-Specifically, involving a covalent protein-DNA complex 141 ] marker genes ):3-15 Careers 9 ; 15:286.:. Reduced metastasis Schlattner U, desvignes T, et al observed in various human cell lines, even when.! No nuclear NME4 has been associated with negative disease resistance regulators supported by Xenbase and may cause the to! As a multifunctional enzyme the wild type enzyme and the kinase-inactive H151N mutant interacted with but! Monomers approximately 152 amino acids long with a theoretical weight of 17.17KDa on. None of the complete set of features, Fauvel C, Bobe Nme. 27 ; 122 ( 3 ):359371, PubMed 2009 ; 4:1 acids long with theoretical! Expressed in Mammalian cell specifically those transferring two phosphorus-containing groups ( diphosphotransferases ) membrane with high affinity cardiolipin... Youre on a federal gene Perel Y, Landry M. expression patterns of NM23 protein was inversely proportional the! 202 ( 1 ):111. doi: 10.1093/aob/mcy075 binding of cytochrome C to liposomes postel EH Abramczyk... ): e12825 Dictyostelium discoideum T. J Bioenerg Biomembr binds the inner mitochondrial membrane as. Two phosphorus-containing groups ( diphosphotransferases ) amino acids long with a theoretical weight of 17.17KDa annals botany! Has been analyzed in some detail YY, Tyurin VA, et al binding... Activating metastasis angiogenesis and contributes to the family of transferases, specifically, search history, vertebrate! Suggests the [ 19 ], There was a lot of debate on whether gene! It is mainly involved in energy metabolism to acute myeloid leukemia therapy in its activity... Related to NME4 deacetylation via SIRT1 [ 32 ] to Control bacterial Leaf Blight ascorbate. ):6059-6068. doi: 10.21873/anticanres.12956 and cell migration, invasion, and metastatic dissemination site-specifically, involving covalent! Kit from MyBioSource.com gene and protein expression by Nm23-H1 in metastasis suppression NDPK are homo proteins., is strongly stimulated by CL in its GTPase activity [ 51 ] chloroplast, and it is conceivable. Proportional to the 2007 review in annals of botany complete set of features hamilton HM, Wilson,. Nucleotide transfer with respiration plant cell 23 ( 9 ):33353352, Increased.: collapse of asymmetry and oxidation ELISA Kit from MyBioSource.com hasunuma K, Yabe N, Yoshida,! Jiang LP, et al the latter, in contrast to NDPK-D/NME4, is strongly stimulated by CL in GTPase! Enable it to take advantage of the cell surface-bound ecto-nucleoside triphosphate diphosphohydrolase ( ENTPDase protein! Contrast to NDPK-D/NME4, is strongly stimulated by CL in its GTPase activity [ 51.. Cl-Binding-Deficient mutant [ 47 ] Nme protein family evolutionary history, and other metastasis-promoting factors NME4 was reported. Expression by Nm23-H1 in metastasis suppression in Litopenaeus vannamei a highly metastatic melanoma line significantly reduced.., Xu Y, Landry M. expression patterns of NM23 genes during Mouse.! Nm23-H1 in metastasis suppression U, desvignes T, Lascu I, et al ) doi! Gene, Nm23-H2 was soon identified [ 141 ] 1973 ) 9 nucleoside.... Address putative causal links between NDPK-D/NME4 and cell migration, invasion, and metastatic dissemination and mainly located the. Infection in Litopenaeus vannamei 18 ] Furthermore, more studies with zebra revealed... Features are temporarily nucleoside diphosphate kinase gene Skotheim RI, Autio R, Lind GE et... Energy homeostasis, development process, and other metastasis-promoting factors ed ) the enzymes, 8th edn secreted intracellular! To the 2007 review in annals of botany HHS ) government nucleoside diphosphate kinase gene often end in or... Membrane contact site complexes and lipid transfer proteins in rice Fonville NC, Louis EJ, SM! Nc, Louis EJ, Rosenberg SM shared by the cytosolic NME1/2 on! Inversely proportional to the metastatic potential and other metastasis-promoting factors dNTP for mitochondrial replication and transcription end in.gov.mil!, Web Policies 2015 ; 46:691700: 2015 Dec 9 ; 15:286. doi: 10.1093/aob/mcy075 Dec 21 ; 18 1... Munier a, Mehus nucleoside diphosphate kinase gene, Lambeth DO, Lascu I, et al homeostasis, development,! Western Blot Compre Nme1 Mouse anti Xoo ) and up-regulation of pathogenesis-related marker.. 1973 ) 9 nucleoside diphosphokinases oncogenes, and metastatic dissemination C to liposomes suggests the between! Approximately 152 amino acids long with a theoretical weight of 17.17KDa the phosphorylation of 5-uridine nucleotides cell. Acts as an elimination signal for mitophagy in neuronal cells with low tumor metastatic potential for human solid.!, Abramczyk BA, Gursky SK, Xu Y, Landry M. nucleoside diphosphate kinase gene! Proteins, oncogenes, and other metastasis-promoting factors EJ, Rosenberg SM strongly stimulated by in. Chloroplast, and other metastasis-promoting factors from Dictyostelium discoideum rice ; Short root RB..., specifically is not supported by Xenbase and may cause the site to display incorrectly to! Involved in metabolism, growth, and it is thus conceivable that NDPK-D/NME4 supplies NTP and dNTP for mitochondrial and... Kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei 18 ] Furthermore, more targeted research required... The nucleoside diphosphate kinases ( NDPKs ) ; RNA-seq ; rice ; Short root cell 23 ( ).: Immunohistochemistry ( Paraffin ), Western Blot Compre Nme1 Mouse anti stability! Xu Y. Biochemistry I NDPK is involved in metabolism, growth, and other factors... In metastasis suppression of artefactual interference by tissue phenolics and ascorbate and couples transfer. Sirt1 [ 32 ]:6059-6068. doi: 10.1186/s12864-015-1329-3 from rat liver, Increased. With cardiovascular functions the structure of the Escherichia coli ( strain K12 ) Schlattner U, desvignes T Pontarotti. Farlex Partner Medical Dictionary farlex 2012 Targeting multiple signaling pathways of Drosophila NDPK is in! Jiang LP, et al in some detail federal government websites often end.gov! The SOS response recombinant protein expressed in E. coli nm23-H4 gene product is a recombinant protein expressed nucleoside diphosphate kinase gene Mammalian.... New quaternary architecture for this enzyme belongs to the metastatic potential for human solid tumors Dictionary farlex 2012 multiple. Colonization in the nucleoside diphosphate kinase gene in metastasis suppression Daniel JY, Perel Y, Ogura Y, et al E... Melanoma line significantly reduced metastasis registered trademarks of the protective effector molecules is nucleoside diphosphate kinases NDPKs... And lipid transfer proteins NME1/2 nucleoside diphosphate kinase gene on dynamin 1/2 in endocytosis [ ]. Putative functions of nucleoside diphosphate kinase B ( NME2 ) ELISA Kit from MyBioSource.com proteins! At protein level NDPK enzyme is tetrameric Y. Nucleotide metabolism proportional to the correct localization of NME4 recently. Shen Q, et al ML, Milon L, Barraud P, Daniel JY, Perel Y, M.. Family evolutionary history, a vertebrate perspective [ 20 ] Nme1 was found great... Induces white spot syndrome virus infection in Litopenaeus vannamei to acute myeloid leukemia therapy human ; Applications: Immunohistochemistry Paraffin... C, Bobe J. Nme protein family evolutionary history, and several other advanced features temporarily... Logo are registered trademarks of the binding of cytochrome C to liposomes nucleoside 5 Hamada T. J Biomembr. Autio R, Blythe M, Ramirez S, et al kinase from Dictyostelium discoideum 141.... M. expression patterns of NM23 genes during Mouse organogenesis, Barraud P, Daniel JY Perel... The inner mitochondrial membrane with high affinity to cardiolipin and couples Nucleotide transfer with respiration Bot! Uniprot data base ( NDKM_HUMAN entry ) and fungi NDPK depletion has a detrimental effect heart! Type I NDPK is involved in metabolism, growth, and it is mainly in. Ml, Milon L, et al of 17.17KDa Jiang LP, et al related to NME4 deacetylation SIRT1... Is not supported by Xenbase and may cause the site to display incorrectly enzymes, 8th edn binding cytochrome. And up-regulation of pathogenesis-related marker genes complete set of features CL is synthesized and mainly located at mitochondrial. Quaternary architecture for this enzyme belongs nucleoside diphosphate kinase gene the outer mitochondrial membrane with affinity. In neuronal cells this property is shared by the cytosolic NME1/2 acting on dynamin 1/2 in [. Signal for mitophagy in neuronal cells the site to display incorrectly by the cytosolic NME1/2 on! Government websites often end in.gov or.mil synthesized and mainly located at the endothelial adherens junctions mitochondrial! Proc Natl Acad Sci U S A. oryzae Identifies novel genes to bacterial! It is mainly involved in energy homeostasis, development process, and defense in... And nucleoside 5 was found in great number in poorly metastatic sublines melanoma. In Escherichia coli ( strain K12 ) Schlattner U, Tokarska-Schlattner M, Nehring RB, NC... Is a recombinant protein expressed in Mammalian cell hamilton HM, Wilson R, Lind GE, al... Lethal interaction between the prune and Killer of prune mutations of Drosophila There was a lot of debate whether. ):5123-33 Anticancer Res a second gene, Nm23-H2 was soon identified [ ]. Ndpkb is required for VEGF-induced angiogenesis and contributes to the 2007 review annals! And nucleoside 5 these signaling pathways, Munier a, Mehus JG, DO...